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Fig. 7 Wnt/β-catenin signaling regulates FGF signaling during fin regeneration. (A) fgf20a expression as detected by semi-quantitative RT-PCR is greatly reduced in Dkk1-overexpressing fins at 3 hpa. Wild-type and hsDkk1GFP transgenic fish were treated according to the schematic (hs, heat shock; amp, amputation; the green line indicates inhibition of Wnt/β-catenin signaling), and RNA was harvested from the tissue adjacent to the amputation plane of nine wild-type and two groups of nine hsDkk1GFP transgenic fins. odc1 amplification serves as a loading control. The experiment was repeated four times using two sets of biological samples and representative results are shown. (B) fgf20a expression is greatly reduced as detected by in situ hybridization in hsDkk1GFP transgenic fins (5 of 6 fins) at 24 hpa as compared with wild-type fins. (C) sprouty4 expression is greatly reduced 6 hours after heat shock in hsDkk1GFP fins (n=3) at 72 hpa.