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Fig. 5 Dual in situ hybridisation histochemistry using probes for both cOpn4x (fluorescein) and cOpn4m (digoxigenin) reveals extensive cOpn4x expression [green; (A)], a more restricted pattern for cOpn4m [red; (B)], and widespread colocalisation of mRNA for the two genes [yellow; (C), merged image] in sections from 2-wk-old chicken retina. Control sense probes showed no nonspecific labelling [(D), cOpn4x; panel E, cOpn4m; (F), merged image]. ONL = outer nuclear; INL = inner nuclear layer; GCL = ganglion cell layer. Scale bar = 50 µm.
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