Fig. 2 Endothelial markers are appropriately expressed in y56/y70 and y55/y66 mutants. A-I: Whole-mount in situ hybridization of mid-trunk vessels in 24 hours postfertilization (hpf) wild-type (A,D,G), y56 mutant (B,E,H), and y66 mutant (C,F,I) embryos using probes for plxnD1 (A-C), flt4 (D-F), and efnb2a (G-I). J: The region shown in the images in A-I is highlighted in red. Endothelial markers are appropriately expressed in mutants, with restriction of efnb2a and flt4 to arterial and venous vessels, respectively. Anterior is to the left and dorsal is up in all panels. The camera lucida drawing in J is modified from Kimmel et al. (1995).
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