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Fig. 5 αA-crystallin overexpression prevents *gamma;-crystallin insolubility. (A) Uninjected embryos. (B) Embryos injected with αA-crystallin mRNA. Zebrafish embryos (2.5 dpf) were lysed detergent-free in 20 mM Tris-HCl (pH 7.5) and centrifuged. Soluble (top) and insoluble (bottom) fractions were separated, and were each analyzed by western blot with anti-γ-crystallin antibody. The intensity of the bands in the western blots was measured by an image analysis program. Three independent experiments using clochem39 were performed. The bar graph represents the mean±s.d.
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