Fig. 4 Expression of mnr2a in the exocrine pancreas. (A–C) Expression of mnr2a at 25 hpf (A), 36 hpf (B) and 96 hpf (C). Indicated are mnr2a expression domains in the endoderm (black arrowhead), in rhombomeres 5, 6 (white arrowhead) and in the spinal cord (sc). (D–J) Double labeling of mnr2a (purple) and insulin (red) expression at 25 hpf (D, E), 33 hpf (F), 36 hpf (G), 48 hpf (H), 70 hpf (I) and 84 hpf (J). Arrows in E, F and G mark mnr2a-positive close to insulin-expressing cells, note that mnr2a and insulin are expressed in different cells; the bar marks the position of the notochord (n). (K) Overlapping expression of mnr2a (blue) and trypsin (red) at 96 hpf. Embryos are shown from lateral (A–C, E) or dorsal (D, F–K) with anterior to the left. Scale bars correspond to 50 μm.
Reprinted from Developmental Biology, 268(2), Wendik, B., Maier, E., and Meyer, D., Zebrafish mnx genes in endocrine and exocrine pancreas formation, 372-383, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.