ZFIN Image: Reugels et al., 2006, Fig. 6

Image

Figure Caption

Fig. 6 A-H: Comparison of Numb(PTB^L PRR^L):EGFP, Numb(PTB^S PRR^L):EGFP, Nbl:EGFP, and five different Numb:EGFP deletion constructs with respect to their localization in dividing cells. Confocal micrographs showing a dorsal view of mitotic cells in the neural tube of embryos injected with the corresponding mRNA. Anterior is towards the top and the midline/apical surface is approximately in the middle of each photograph. See text for details. B-D: Note that the nucleus in non-dividing cells is free of the signal (arrow), whereas in mitotic cells (arrowhead) the signal is found in the entire cell, after the nuclear membrane has broken down. F: Amino acids 1-196 (including the PTB^L insertion) are efficiently localized to the basolateral cortex. G: Deletion of amino acids 2-12 impairs cortical and abolishes basolateral localization in dividing cells. H: Impairment of cortical localization is enhanced after deletion of amino acids 2-22.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.