|
Fig. 8 Developmental expression analysis of zebrafish grna and grnb mRNAs by whole mount in situ hybridization. An ontogeny of expression conducted for grna (A) and grnb (B) revealed similar expression patterns for these genes from fertilization to late segmentation stage (a-f), with grna being weaker than grnb. At the 4-cell stage (a) and 50% epiboly (b) ubiquitous expression is observed for grnb only. A lateral view of the 6-somite stage embryo (c) reveals discernable ubiquitous expression above background levels for grna, and increased grnb expression in the epithelium of the eye primordium and CNS, as well as the caudal region. In a dorsal view of the same animals (d), caudal expression in the axial mesoderm (arrow) is observed for both genes, whereas only grnb is detected in the paraxial mesoderm (arrowheads). Lateral (e) and frontal (f) views of the late somitogenesis stage embryo (18-20 hpf) show continued expression in the eye primordium, CNS and tailbud for both genes. In addition, grnb can be detected in the YSL (arrow) (e) and the adaxial cells (arrowheads) (f) flanking the axial mesoderm. At 24 hpf (g), grna expression is found in the tectum and eye retina, in a diffuse pattern in the anterior endoderm (arrowhead) and in a punctuate pattern within the ventral tail region of the ICM (arrow), whereas elevated expression persists for grnb in the forebrain, midbrain and ventral hindbrain region, the eyes, as well as in the YSL, concentrated at the tip of the yolk extension (arrow). In a lateral view at 48 hpf (h), grna expression in the ICM extends rostrally, is detected in the head vasculature, and is now apparent in the skin epithelium, whereas in a lateral view (i) both grna and grnb are transiently expressed in the AER of the pectoral fin buds (arrows). At 72 hpf (j), grna, but not grnb, is expressed in presumed dispersed leukocytes, while in a dorsolateral view (k), grnb can be detected in the swim bladder (arrow). AE, anterior endoderm; AER, apical ectodermal ridge of the pectoral fin buds; ICM, intermediate cell mass; LPM. lateral plate mesoderm; YSL, yolk syncytial layer.