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Fig. S1 pitx3 mRNA translation is blocked by pitx3 morpholinos. (A-D) pitx3 mRNA rescues pitx3 morpholino-injected embryos (8 ng morpholino/embryo). (E-G) Embryos co-injected with increasing doses of pitx3 morpholino and pitx3:eGFP mRNA (mRNA from 5′UTR of pitx3 cDNA linked to eGFP; 300 pg/embryo). Morpholinos suppress eGFP fluorescence in a dose-dependent manner (F,G). By contrast, eGFP fluorescence is not blocked by pitx3 morpholino in embryos injected with a modified mRNA construct 5Dpitx3:egfp that contains five mismatches in the morpholino-binding site of the pitx3 5′ UTR (I). (A-D) Frontal view, dorsal towards the top; (E-I) Side views, animal pole towards top. Scale bars: 200 μm in A-D; 100 μm in E-I.