Et(GAL4-VP16,myl7:GFP)

ID

ZDB-ETCONSTRCT-120604-1

Name

Et(GAL4-VP16,myl7:GFP)

Previous Names

• Et(Gal4-VP16)
• Et(bp:Gal4VP16) (1)
• Et(Gal4-VP16,myl7:GFP)

Type

engineered_plasmid

Regulatory Regions

• myl7 (1)

Coding Sequences

• GAL4 (1)
• GFP (1)
• VP16 (1)

Contains Sequences

None

Note

This enhancer trapping construct is based on pDestTol2CG3, a multisite-Gateway-based plasmid. It contains a minimal adenovirus E1b promoter driving Gal4VP16 (Gal4 DNA-binding domain fused to the VP16 transcriptional activation domain) followed by the late SV40 polyA signal sequence. Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving EGFP followed by the SV40 late polyA signal sequence; this marks the heart with green fluorescence. The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions. The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).