EST

cb501

ID
ZDB-EST-021126-18
Name
cb501
Symbol
cb501
Previous Names
None
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
oligo-dT primed 26-somite stage
Digest
PCR amplification
Insert Size
1400
Cloning Site
EcoRI-SalI
Vector Type
Plasmid
Vector
pSPORT1
Polymerase
T3 RNA polymerase
PCR Amplification
(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Suppliers
Quality
( Nice strong expression pattern )
Protocol
Thisse in situ hybridization protocol
Note
The following is from Rattner A, Smallwood PM, Nathans J. (2000) J Biol Chem 2000 Apr 14;275(15):11034-43
prRDH is the enzyme responsible for the reduction of all-trans-retinal to all-trans-retinol within the photoreceptor outer segment.
Gene Expression
Directly Submitted Expression Data
6 figures (24 images) from Thisse et al., 2001 [cb501]
Marker Relationships
Sequences
Citations