CRISPR

CRISPR1-dldh

ID

ZDB-CRISPR-241209-1

Name

CRISPR1-dldh

Previous Names

None

Target

dldh (1)

Sequence

5' - ATGAGGGTATCATTTGTGTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
cri3	dldh

Expression

Gene expression in Wild Types + CRISPR1-dldh

No data available

Phenotype

Phenotype resulting from CRISPR1-dldh

No data available

Phenotype of all Fish created by or utilizing CRISPR1-dldh

Phenotype	Fish	Conditions	Figures
swimming decreased occurrence, ameliorated	dldh^cri3/cri3 (AB)	low light intensity, chemical treatment by environment: probucol	Fig. 9 from Lavorato et al., 2024
swim bladder uninflated, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 4 , Fig. 5 from Lavorato et al., 2024
whole organism 3-methyl-2-oxobutanoic acid increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
liver mitochondrion damaged, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 5 , Fig. 6 from Lavorato et al., 2024
swimming decreased occurrence, ameliorated	dldh^cri3/cri3 (AB)	low light intensity, chemical treatment by environment: thiamine(1+)	Fig. 9 from Lavorato et al., 2024
whole organism lysine increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
dihydrolipoyl dehydrogenase activity decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 3 from Lavorato et al., 2024
liver increased size, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 4 , Fig. 5 from Lavorato et al., 2024
whole organism lactate increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
intestine mitochondrion elongated, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 7 from Lavorato et al., 2024
oxoglutarate dehydrogenase (succinyl-transferring) activity decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
whole organism dldh expression decreased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 1 from Lavorato et al., 2024
whole organism leucine increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
whole organism viability, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 4 from Lavorato et al., 2024
liver lipid droplet increased size, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 5 from Lavorato et al., 2024
intestine mitochondrion swollen, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 7 from Lavorato et al., 2024
2-oxoadipate reductase activity decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
whole organism vdac1 expression increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 1 from Lavorato et al., 2024
liver lipid droplet increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 5 from Lavorato et al., 2024
fast muscle cell mitochondrion damaged, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 8 from Lavorato et al., 2024
whole organism 2-oxoglutarate(2-) increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
swimming decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 9 from Lavorato et al., 2024
intestine mitochondrion damaged, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 7 from Lavorato et al., 2024
branched-chain alpha-keto acid dehydrogenase activity decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
intestine low brightness, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 4 from Lavorato et al., 2024
pyruvate dehydrogenase activity decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
liver mitochondrion degenerate, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 6 from Lavorato et al., 2024
whole organism valine increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
swimming decreased occurrence, abnormal	dldh^cri3/cri3 (AB)	low light intensity	Fig. 4 from Lavorato et al., 2024
intestine mitochondrion decreased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 7 from Lavorato et al., 2024
liver mitochondrion shape, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 6 from Lavorato et al., 2024
whole organism glutarate increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
fast muscle cell mitochondrion shape, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 8 from Lavorato et al., 2024
whole organism isoleucine increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 2 from Lavorato et al., 2024
liver lipid increased amount, abnormal	dldh^cri3/cri3 (AB)	standard conditions	Fig. 5 from Lavorato et al., 2024
whole organism vdac1 expression increased amount, abnormal	dldh^cri3/+ (AB)	standard conditions	Fig. 1 from Lavorato et al., 2024

Citations