CRISPR

CRISPR8-nrg1

ID

ZDB-CRISPR-240119-7

Name

CRISPR8-nrg1

Previous Names

None

Target

Sequence

5' - GGAGCTCGCCCGCCGCTCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
st152	nrg1

Expression

Gene expression in Wild Types + CRISPR8-nrg1

No data available

Phenotype

Phenotype resulting from CRISPR8-nrg1

Phenotype	Fish	Figures
oligodendrocyte myelin sheath decreased amount, abnormal	ue101Tg/ue101Tg; ue102Tg/ue102Tg + CRISPR8-nrg1	Fig. 5 from Lysko et al., 2022
Rohon-Beard neuron decreased amount, abnormal	ue101Tg/ue101Tg; ue102Tg/ue102Tg + CRISPR8-nrg1	Fig. 5 from Lysko et al., 2022

Phenotype of all Fish created by or utilizing CRISPR8-nrg1

Phenotype	Fish	Conditions	Figures
reticulospinal neuron myelin sheath decreased amount, abnormal	nrg1^st152/st152	standard conditions	Fig. 3 from Lysko et al., 2022
oligodendrocyte myelin sheath decreased amount, abnormal	nrg1^st152/st152	standard conditions	Fig. 2 from Lysko et al., 2022
CoPA myelin sheath decreased amount, abnormal	nrg1^st152/st152	standard conditions	Fig. 3 from Lysko et al., 2022
spinal cord myelin sheath GFP expression decreased amount, abnormal	nrg1^st152/+; ue101Tg/ue101Tg; ue102Tg/ue102Tg	standard conditions	Fig. 1 from Lysko et al., 2022
spinal cord myelin sheath GFP expression decreased amount, abnormal	nrg1^st152/st152; ue101Tg/ue101Tg; ue102Tg/ue102Tg	standard conditions	Fig. 1 from Lysko et al., 2022
oligodendrocyte myelin sheath decreased amount, abnormal	ue101Tg/ue101Tg; ue102Tg/ue102Tg + CRISPR8-nrg1	standard conditions	Fig. 5 from Lysko et al., 2022
Rohon-Beard neuron decreased amount, abnormal	ue101Tg/ue101Tg; ue102Tg/ue102Tg + CRISPR8-nrg1	standard conditions	Fig. 5 from Lysko et al., 2022

Citations