CRISPR

CRISPR2-spi2

ID

ZDB-CRISPR-231130-2

Name

CRISPR2-spi2

Previous Names

None

Target

Sequence

5' - GGGCTATAATTTCGCAAGGCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first "G" was added. The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
hkz58	spi2

Expression

Gene expression in Wild Types + CRISPR2-spi2

No data available

Phenotype

Phenotype resulting from CRISPR2-spi2

No data available

Phenotype of all Fish created by or utilizing CRISPR2-spi2

Phenotype	Fish	Conditions	Figures
neutrophil decreased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 from Zhao et al., 2022
macrophage decreased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 from Zhao et al., 2022
caudal hematopoietic tissue myb expression decreased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 4 from Zhao et al., 2022
caudal hematopoietic tissue myb expression decreased distribution, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 , Fig. 4 from Zhao et al., 2022
nucleate erythrocyte hbae1.1 expression decreased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 from Zhao et al., 2022
hematopoietic multipotent progenitor cell cell division decreased frequency, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 4 from Zhao et al., 2022
hematopoietic multipotent progenitor cell increased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 from Zhao et al., 2022
myeloblast increased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 from Zhao et al., 2022
myeloid cell decreased amount, abnormal	spi2^hkz58/hkz58	standard conditions	Fig. 3 from Zhao et al., 2022
macrophage DsRed expression decreased amount, abnormal	spi2^hkz58/hkz58; hkz015tTg/hkz015tTg	standard conditions	Fig. 3 from Zhao et al., 2022
ventral wall of dorsal aorta neutrophil DsRed expression decreased amount, abnormal	spi2^hkz58/hkz58; nz50Tg/nz50Tg	standard conditions	Fig. 3 from Zhao et al., 2022

Citations