CRISPR

CRISPR1-lratd2a

ID

ZDB-CRISPR-230828-1

Name

CRISPR1-lratd2a

Previous Names

None

Target

lratd2a (1)

Sequence

5' - CTGGACACCGAAGAAGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGA" at the 5' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
c601Tg	lratd2a

Expression

Gene expression in Wild Types + CRISPR1-lratd2a

No data available

Phenotype

Phenotype resulting from CRISPR1-lratd2a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-lratd2a

Phenotype	Fish	Conditions	Figures
dorsal habenular nucleus right side GCaMP expression increased amount, abnormal	lratd2a^{c601Tg/c601Tg}; c587Tg/c587Tg	chemical treatment by environment: chondroitin sulfate	Figure 2 from Choi et al., 2021
dorsal habenular nucleus calcium ion import increased rate, abnormal	lratd2a^{c601Tg/c601Tg}; c587Tg/c587Tg	chemical treatment by environment: chondroitin sulfate	Figure 2 from Choi et al., 2021
dorsal habenular nucleus right side GCaMP expression increased amount, abnormal	lratd2a^{c601Tg/c601Tg}; c587Tg/c587Tg	chemical treatment by environment: cadaverine	Figure 2 from Choi et al., 2021
dorsal habenular nucleus calcium ion import increased rate, abnormal	lratd2a^{c601Tg/c601Tg}; c587Tg/c587Tg	chemical treatment by environment: cadaverine	Figure 2 from Choi et al., 2021
dorsal habenular nucleus GFP expression spatial pattern, abnormal	bsx^m1376/+; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf15Tg/zf15Tg	standard conditions	Figure 5 from Choi et al., 2021
dorsal habenular nucleus GFP expression spatial pattern, abnormal	bsx^m1376/m1376; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf15Tg/zf15Tg	standard conditions	Figure 5 from Choi et al., 2021
dorsal habenular nucleus axon mApple expression spatial pattern, abnormal	bsx^m1376/m1376; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf15Tg/zf15Tg	standard conditions	Figure 5 from Choi et al., 2021
dorsal habenular nucleus right side kctd12.1 expression decreased amount, abnormal	tcf7l2^zf55/+; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf177Tg/zf177Tg	standard conditions	Figure 4 from Choi et al., 2021
interpeduncular nucleus tegmentum mApple expression decreased amount, abnormal	tcf7l2^zf55/zf55; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg	standard conditions	Figure 4 from Choi et al., 2021
olfactory bulb lratd2a expression decreased amount, abnormal	tcf7l2^zf55/zf55; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf177Tg/zf177Tg	standard conditions	Figure 4 from Choi et al., 2021
dorsal habenular nucleus right side lratd2a expression decreased amount, abnormal	tcf7l2^zf55/zf55; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf177Tg/zf177Tg	standard conditions	Figure 4 from Choi et al., 2021
dorsal habenular nucleus right side EYFP expression decreased amount, abnormal	tcf7l2^zf55/zf55; lratd2a^{c601Tg/c601Tg}; c636Tg/c636Tg; zf177Tg/zf177Tg	standard conditions	Figure 4 from Choi et al., 2021
response to stress decreased process quality, abnormal	lratd2a^{c601Tg/c601Tg}; slc5a7a^{c662Tg/c662Tg}; c674Tg/c674Tg	chemical treatment by environment: cadaverine	Figure 3 from Choi et al., 2021
swimming behavior decreased process quality, abnormal	lratd2a^{c601Tg/c601Tg}; slc5a7a^{c662Tg/c662Tg}; c674Tg/c674Tg	chemical treatment by environment: cadaverine	Figure 3 from Choi et al., 2021
dorsal habenular nucleus chemical synaptic transmission decreased process quality, abnormal	lratd2a^{c601Tg/c601Tg}; slc5a7a^{c662Tg/c662Tg}; c674Tg/c674Tg	chemical treatment by environment: chondroitin sulfate	Figure 3 from Choi et al., 2021
dorsal habenular nucleus chemical synaptic transmission decreased process quality, abnormal	lratd2a^{c601Tg/c601Tg}; slc5a7a^{c662Tg/c662Tg}; c674Tg/c674Tg	chemical treatment by environment: cadaverine	Figure 3 from Choi et al., 2021
dorsal habenular nucleus right side GFP expression decreased amount, abnormal	lratd2a^{c601Tg/c601Tg}; slc5a7a^{c662Tg/c662Tg}; c674Tg/c674Tg	standard conditions	Figure 3 from Choi et al., 2021

Citations