CRISPR

CRISPR1-hcn2b

ID

ZDB-CRISPR-230809-3

Name

CRISPR1-hcn2b

Previous Names

None

Target

Sequence

5' - CGCGGGAGGAGCGGCGGCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
una101	hcn2b

Expression

Gene expression in Wild Types + CRISPR1-hcn2b

No data available

Phenotype

Phenotype resulting from CRISPR1-hcn2b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-hcn2b

Phenotype	Fish	Conditions	Figures
post-vent region motor behavior increased process quality, abnormal	hcn2b^{una101/una101}	standard conditions	Figure 3 from Rodríguez-Ortiz et al., 2021
swimming behavior increased process quality, abnormal	hcn2b^{una101/una101}	altered light dark cycle	Figure 4 from Rodríguez-Ortiz et al., 2021
swimming behavior increased process quality, abnormal	hcn2b^{una101/una101}	control	Figure 6 from Rodríguez-Ortiz et al., 2021
swimming behavior increased process quality, abnormal	hcn2b^{una101/una101}	light intensity	Figure 5 from Rodríguez-Ortiz et al., 2021
whole organism viability, abnormal	hcn2b^{una101/una101}	standard conditions	Figure 2 from Rodríguez-Ortiz et al., 2021
heart contraction increased rate of occurrence, abnormal	hcn2b^{una101/una101}	standard conditions	Figure 2 from Rodríguez-Ortiz et al., 2021

Citations