CRISPR

CRISPR1-nphp4

ID

ZDB-CRISPR-230307-4

Name

CRISPR1-nphp4

Previous Names

None

Target

Sequence

5' - GGTCTTTCAGTGTTGGGTGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uab21	nphp4

Expression

Gene expression in Wild Types + CRISPR1-nphp4

No data available

Phenotype

Phenotype resulting from CRISPR1-nphp4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-nphp4

Phenotype	Fish	Conditions	Figures
posterior lateral line neuromast decreased amount, abnormal	nphp4^uab21/uab21 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
anterior lateral line neuromast decreased amount, abnormal	nphp4^uab21/uab21 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
vertebral column rotational curvature, abnormal	nphp4^uab21/uab21; bbs5^uab22/uab22 (AB)	standard conditions	Fig. 4 from Bentley-Ford et al., 2021
photoreceptor inner segment layer disorganized, abnormal	nphp4^uab21/uab21; bbs5^uab22/uab22 (AB)	standard conditions	Fig. 4 from Bentley-Ford et al., 2021
vertebral column increased curvature, abnormal	nphp4^uab21/uab21; bbs5^uab22/uab22 (AB)	standard conditions	Fig. 4 from Bentley-Ford et al., 2021
photoreceptor inner segment layer decreased thickness, abnormal	nphp4^uab21/uab21; bbs5^uab22/uab22 (AB)	standard conditions	Fig. 4 from Bentley-Ford et al., 2021
photoreceptor outer segment layer disorganized, abnormal	nphp4^uab21/uab21; bbs5^uab22/uab22 (AB)	standard conditions	Fig. 4 from Bentley-Ford et al., 2021
photoreceptor outer segment layer decreased thickness, abnormal	nphp4^uab21/uab21; bbs5^uab22/uab22 (AB)	standard conditions	Fig. 4 from Bentley-Ford et al., 2021

Citations