CRISPR

CRISPR1-cc2d2a

ID

ZDB-CRISPR-230306-3

Name

CRISPR1-cc2d2a

Previous Names

None

Target

Sequence

5' - AGTGTCTGAGTGTCTCACCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uab16	cc2d2a

Expression

Gene expression in Wild Types + CRISPR1-cc2d2a

No data available

Phenotype

Phenotype resulting from CRISPR1-cc2d2a

Phenotype	Fish	Figures
post-vent region increased curvature, abnormal	AB + CRISPR1-cc2d2a	Fig. 7. from Wang et al., 2022

Phenotype of all Fish created by or utilizing CRISPR1-cc2d2a

Phenotype	Fish	Conditions	Figures
retinal rod cell photoreceptor inner segment disorganized, abnormal	cc2d2a^uab16/uab16 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
vertebral column increased curvature, abnormal	cc2d2a^uab16/uab16 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
retinal cone cell photoreceptor outer segment decreased amount, abnormal	cc2d2a^uab16/uab16 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
whole organism dead, abnormal	cc2d2a^uab16/uab16 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
post-vent region curved ventral, abnormal	cc2d2a^uab16/uab16 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
anterior lateral line neuromast amount, ameliorated	cc2d2a^uab16/uab16 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
posterior lateral line neuromast amount, ameliorated	cc2d2a^uab16/uab16 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
post-vent region increased curvature, abnormal	AB + CRISPR1-cc2d2a	standard conditions	Fig. 7. from Wang et al., 2022
post-vent region curved ventral, abnormal	AB + CRISPR1-cc2d2a + CRISPR2-cc2d2a + CRISPR3-cc2d2a	standard conditions	Fig. 7. from Wang et al., 2022
post-vent region increased curvature, abnormal	AB + CRISPR1-cc2d2a + CRISPR2-cc2d2a + CRISPR3-cc2d2a	standard conditions	Fig. 7. from Wang et al., 2022

Citations