CRISPR

CRISPR2-b9d2

ID

ZDB-CRISPR-230306-23

Name

CRISPR2-b9d2

Previous Names

None

Target

b9d2 (1)

Sequence

5' - GGACAGATCATTGGGGCCAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uab17	b9d2

Expression

Gene expression in Wild Types + CRISPR2-b9d2

No data available

Phenotype

Phenotype resulting from CRISPR2-b9d2

No data available

Phenotype of all Fish created by or utilizing CRISPR2-b9d2

Phenotype	Fish	Conditions	Figures
vertebral column rotational curvature, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 2. from Wang et al., 2022
male organism male sterile, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	text only from Wang et al., 2022
retinal rod cell photoreceptor inner segment disorganized, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
retinal cone cell photoreceptor outer segment decreased amount, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
head increased angle to trunk, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
retinal cone cell decreased amount, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
anterior lateral line neuromast amount, ameliorated	b9d2^uab17/uab17 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
posterior lateral line neuromast amount, ameliorated	b9d2^uab17/uab17 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
retinal outer nuclear layer cell population proliferation increased occurrence, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
retina inflammatory response increased occurrence, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
vertebral column increased curvature, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 1. , Fig. 2. from Wang et al., 2022
female organism female sterile, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	text only from Wang et al., 2022
post-vent region curved ventral, abnormal	b9d2^uab17/uab17 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
vertebral column rotational curvature, abnormal	AB + CRISPR1-b9d2 + CRISPR2-b9d2 + CRISPR3-b9d2 + CRISPR4-b9d2	standard conditions	Fig. 7. from Wang et al., 2022
vertebral column increased curvature, abnormal	AB + CRISPR1-b9d2 + CRISPR2-b9d2 + CRISPR3-b9d2 + CRISPR4-b9d2	standard conditions	Fig. 7. from Wang et al., 2022

Citations