CRISPR

CRISPR5-myh9b

ID

ZDB-CRISPR-221208-3

Name

CRISPR5-myh9b

Previous Names

None

Target

Sequence

5' - CTGGATAAGACCAAGCGTCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3748	myh9b

Expression

Gene expression in Wild Types + CRISPR5-myh9b

No data available

Phenotype

Phenotype resulting from CRISPR5-myh9b

No data available

Phenotype of all Fish created by or utilizing CRISPR5-myh9b

Phenotype	Fish	Conditions	Figures
cardiac muscle Z disc aggregated, abnormal	bag3^xu083/xu083 + CRISPR5-myh9b	standard conditions	Fig. 6 from Ding et al., 2022
cardiac ventricle increased size, exacerbated	bag3^xu083/xu083 + CRISPR5-myh9b	standard conditions	Fig. 6 from Ding et al., 2022
cardiac muscle myoblast mitochondrion swollen, abnormal	bag3^xu083/xu083 + CRISPR5-myh9b	standard conditions	Fig. 6 from Ding et al., 2022
heart contraction decreased efficacy, exacerbated	bag3^xu083/xu083 + CRISPR5-myh9b	standard conditions	Fig. 6 from Ding et al., 2022
cardiac muscle Z disc aggregated, abnormal	bag3^xu083/xu083; myh9b^zf3748/+	standard conditions	Fig. 7 from Ding et al., 2022
cardiac ventricle anatomical surface increased area, exacerbated	bag3^xu083/xu083; myh9b^zf3748/+	standard conditions	Fig. 7 from Ding et al., 2022
heart Ab11-map1lc3 labeling decreased amount, abnormal	bag3^xu083/xu083; myh9b^zf3748/+	standard conditions	Fig. 7 from Ding et al., 2022
heart contraction decreased efficacy, exacerbated	bag3^xu083/xu083; myh9b^zf3748/+	standard conditions	Fig. 7 from Ding et al., 2022

Citations