CRISPR

CRISPR2-irx1b

ID

ZDB-CRISPR-221201-4

Name

CRISPR2-irx1b

Previous Names

None

Target

irx1b (1)

Sequence

5' - GCGTGCACTGCAAATCCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
el833	irx1b

Expression

Gene expression in Wild Types + CRISPR2-irx1b

No data available

Phenotype

Phenotype resulting from CRISPR2-irx1b

No data available

Phenotype of all Fish created by or utilizing CRISPR2-irx1b

Phenotype	Fish	Conditions	Figures
quadrate fused with anguloarticular, abnormal	irx1b^el833/el833; irx4b^el834/el834	standard conditions	Fig. 6 from Farmer et al., 2021
Meckel's cartilage fused with ceratohyal bone, abnormal	irx1b^el833/el833; irx4b^el834/el834	standard conditions	Fig. 6 from Farmer et al., 2021
palatoquadrate cartilage absent, abnormal	irx1b^el833/el833; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
ceratohyal bone fused with ceratobranchial 1 bone, abnormal	irx1b^el833/el833; irx4b^el834/el834	standard conditions	Fig. 6 from Farmer et al., 2021
Meckel's cartilage absent, abnormal	irx1b^el833/el833; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
pharyngeal arch 3-7 cranial neural crest cell decreased amount, abnormal	irx1b^el833/el833; irx7^el540/el540; irx4b^el834/el834	standard conditions	Fig. 4 from Farmer et al., 2021
neural crest cell dlx2a expression decreased amount, abnormal	irx1b^el833/el833; irx7^el540/el540; irx4b^el834/el834	standard conditions	Fig. 4 from Farmer et al., 2021
cranial neural crest cell sox10 expression decreased amount, abnormal	irx1b^el833/el833; irx7^el540/el540; irx4b^el834/el834	standard conditions	Fig. 4 from Farmer et al., 2021
pharyngeal arch 1 cranial neural crest cell absent, abnormal	irx1b^el833/el833; irx7^el540/el540; irx4b^el834/el834; el10Tg	standard conditions	Fig. 3 from Farmer et al., 2021
pharyngeal arch 2 cranial neural crest cell decreased amount, abnormal	irx1b^el833/el833; irx7^el540/el540; irx4b^el834/el834; el10Tg	standard conditions	Fig. 3 from Farmer et al., 2021
axis curved dorsal, abnormal	irx1b^el833/el833; irx1a^el830/el830; irx2a^el831/el831; irx4a^el832/el832; irx4b^el834/el834	standard conditions	Fig. 1 from Farmer et al., 2021
Meckel's cartilage absent, abnormal	irx1b^el833/el833; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
palatoquadrate cartilage absent, abnormal	irx1b^el833/el833; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
ceratobranchial cartilage absent, abnormal	irx1b^el833/el833; irx3a^el835/el835; irx3b^el837/el837; irx5a^el576/el576; irx5b^el722/el722; irx6a^el836/el836; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
ceratobranchial cartilage absent, abnormal	irx1b^el833/el833; irx3a^el835/el835; irx3b^el837/el837; irx5a^el576/el576; irx5b^el722/el722; irx6a^el836/el836; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
palatoquadrate cartilage absent, abnormal	irx1b^el833/el833; irx3a^el835/el835; irx3b^el837/el837; irx5a^el576/el576; irx5b^el722/el722; irx6a^el836/el836; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
Meckel's cartilage absent, abnormal	irx1b^el833/el833; irx3a^el835/el835; irx3b^el837/el837; irx5a^el576/el576; irx5b^el722/el722; irx6a^el836/el836; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
neurocranium absent, abnormal	irx1b^el833/el833; irx3a^el835/el835; irx3b^el837/el837; irx5a^el576/el576; irx5b^el722/el722; irx6a^el836/el836; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021
ceratohyal cartilage decreased size, abnormal	irx1b^el833/el833; irx3a^el835/el835; irx3b^el837/el837; irx5a^el576/el576; irx5b^el722/el722; irx6a^el836/el836; irx7^el540/el540; irx1a^el830/+; irx2a^el831/+; irx4a^el832/+; irx4b^el834/el834	standard conditions	Fig. 3 from Farmer et al., 2021

Citations