CRISPR

CRISPR2-col22a1

ID

ZDB-CRISPR-221104-3

Name

CRISPR2-col22a1

Previous Names

None

Target

col22a1 (1)

Sequence

5' - GGATGGCGAGAACAGGGCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3608	col22a1

Expression

Gene expression in Wild Types + CRISPR2-col22a1

No data available

Phenotype

Phenotype resulting from CRISPR2-col22a1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-col22a1

Phenotype	Fish	Conditions	Figures
swimming behavior decreased process quality, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	control	Fig. 6 from Malbouyres et al., 2022
trunk col21a1 expression increased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
trunk itga7 expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
whole organism semi-viable, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 2 from Malbouyres et al., 2022
vertical myoseptum fibroblast increased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 4 from Malbouyres et al., 2022
feeding behavior decreased process quality, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 2 from Malbouyres et al., 2022
skeletal muscle mitochondrion increased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	water flow	Fig. 8 from Malbouyres et al., 2022
trunk skeletal muscle cell disorganized, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	electric field	Fig. 4 from Malbouyres et al., 2022
whole organism viability, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 2 from Malbouyres et al., 2022
trunk scxb expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
trunk thbs4b expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
trunk tnmd expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
vertical myoseptum fibrillar collagen trimer area density, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022
skeletal muscle muscle tendon junction morphology, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022
trunk lama4 expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
trunk scxa expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
myoseptum fibroblast increased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022
oxygen metabolic process increased process quality, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	water flow	Fig. 8 from Malbouyres et al., 2022
skeletal muscle muscle tendon junction morphology, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	water flow	Fig. 8 from Malbouyres et al., 2022
whole organism col22a1 expression decreased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 2 from Malbouyres et al., 2022
skeletal muscle sarcolemma decreased length, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022
vertical myoseptum distended, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022
swimming decreased process quality, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	water flow	Fig. 7 from Malbouyres et al., 2022
vertical myoseptum fibrillar collagen trimer organization quality, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022
trunk col1a1a expression increased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
myoseptum col22a1 expression absent, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 1, Fig. 4 from Malbouyres et al., 2022
whole organism decreased length, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 2 from Malbouyres et al., 2022
trunk fn1b expression increased amount, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 5 from Malbouyres et al., 2022
skeletal muscle contraction decreased process quality, abnormal	col22a1^{zf3608/zf3608} (AB/TU)	standard conditions	Fig. 3 from Malbouyres et al., 2022

Citations