CRISPR

CRISPR3-rag2

ID

ZDB-CRISPR-221025-2

Name

CRISPR3-rag2

Previous Names

None

Target

rag2 (1)

Sequence

5' - GGCCCTTGACTACATATGGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end. There is also a single nucleotide difference between this CRISPR and the sequence in Ensemble with the CRISPR ending in GTG and Ensembl with CTG.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
fb106	rag2

Expression

Gene expression in Wild Types + CRISPR3-rag2

No data available

Phenotype

Phenotype resulting from CRISPR3-rag2

No data available

Phenotype of all Fish created by or utilizing CRISPR3-rag2

Phenotype	Fish	Conditions	Figures
head kidney B cell decreased amount, abnormal	rag2^fb106/fb106	standard conditions	Figure 1. from Yan et al., 2021
head kidney T cell decreased amount, abnormal	rag2^fb106/fb106	standard conditions	Figure 1. from Yan et al., 2021
head kidney natural killer cell decreased amount, abnormal	rag2^fb106/fb106	standard conditions	Figure 1. from Yan et al., 2021
head kidney natural killer cell absent, abnormal	il2rga^fb104/fb104; rag2^fb106/fb106	standard conditions	Figure 1. from Yan et al., 2021
head kidney B cell absent, abnormal	il2rga^fb104/fb104; rag2^fb106/fb106	standard conditions	Figure 1. from Yan et al., 2021
head kidney T cell absent, abnormal	il2rga^fb104/fb104; rag2^fb106/fb106	standard conditions	Figure 1. from Yan et al., 2021
whole organism carcinoma present, abnormal	il2rga^fb104/fb104; rag2^fb106/fb106	cancer xenotransplantation	Fig. 3 from Zhang et al., 2021

Citations