CRISPR

CRISPR1-lmo2

ID

ZDB-CRISPR-220825-2

Name

CRISPR1-lmo2

Previous Names

None

Target

Sequence

5' - GCTGATCTGCAGGGAGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3558	lmo2

Expression

Gene expression in Wild Types + CRISPR1-lmo2

No data available

Phenotype

Phenotype resulting from CRISPR1-lmo2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-lmo2

Phenotype	Fish	Conditions	Figures
whole organism prdm16 expression decreased amount, abnormal	lmo2^{zf3558/zf3558}	standard conditions	Fig. 5 from Matrone et al., 2021
endothelial cell prdm16 expression decreased amount, abnormal	lmo2^{zf3558/zf3558}	standard conditions	Fig. 5 from Matrone et al., 2021
whole organism viability, abnormal	lmo2^{zf3558/zf3558}	standard conditions	Fig. 5 from Matrone et al., 2021
intersegmental vessel mCherry expression decreased amount, abnormal	lmo2^{zf3558/zf3558}; y171Tg/y171Tg; y1Tg/y1Tg	standard conditions	Fig. 5 from Matrone et al., 2021
blood vessel endothelial cell EGFP expression decreased amount, abnormal	lmo2^{zf3558/zf3558}; y171Tg/y171Tg; y1Tg/y1Tg	standard conditions	Fig. 5 from Matrone et al., 2021
blood vessel endothelial cell mCherry expression decreased amount, abnormal	lmo2^{zf3558/zf3558}; y171Tg/y171Tg; y1Tg/y1Tg	standard conditions	Fig. 5 from Matrone et al., 2021
intersegmental vessel EGFP expression decreased amount, abnormal	lmo2^{zf3558/zf3558}; y171Tg/y171Tg; y1Tg/y1Tg	standard conditions	Fig. 5 from Matrone et al., 2021
intersegmental vessel decreased length, abnormal	lmo2^{zf3558/zf3558}; y171Tg; y1Tg	standard conditions	Fig. 5 from Matrone et al., 2021
intersegmental vessel decreased amount, abnormal	lmo2^{zf3558/zf3558}; y171Tg; y1Tg	standard conditions	Fig. 5 from Matrone et al., 2021

Citations