CRISPR

CRISPR2-snai1b

ID

ZDB-CRISPR-220427-2

Name

CRISPR2-snai1b

Previous Names

None

Target

snai1b (1)

Sequence

5' - GTAGTTTGGCTTCTTGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Targeting exon 1 of snai1b.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
bns351	snai1b

Expression

Gene expression in Wild Types + CRISPR2-snai1b

No data available

Phenotype

Phenotype resulting from CRISPR2-snai1b

No data available

Phenotype of all Fish created by or utilizing CRISPR2-snai1b

Phenotype	Fish	Conditions	Figures
cardiac muscle cell separated from myocardium, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021
heart trabecula formation decreased process quality, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 4 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021
trabecular layer of ventricle has fewer parts of type cardiac muscle cell, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 4 from Gentile et al., 2021
heart desmb expression increased amount, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 3 from Gentile et al., 2021
heart looping decreased process quality, abnormal	snai1b^{bns351/bns351}; bns193Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021
cardiac muscle cell basal part of cell Ab4-ctnna labeling increased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
cardiac muscle cell basal part of cell ab1-myl-ser20 labeling increased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
cardiac muscle cell cell-cell junction ab1-desm labeling decreased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 3 from Gentile et al., 2021
cardiac muscle cell apical part of cell decreased area, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 1 supplement 5 from Gentile et al., 2021
cardiac ventricle decreased volume, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 1 supplement 5 from Gentile et al., 2021
cardiac muscle cell regulation of cell-cell adhesion mediated by cadherin decreased process quality, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
heart contraction decreased frequency, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 1 supplement 5 from Gentile et al., 2021
cardiac muscle cell basal part of cell ab1-desm labeling increased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 3 from Gentile et al., 2021
cardiac muscle cell cell-cell junction Ab12-cdh2 labeling decreased amount, abnormal	snai1b^{bns351/bns351}; s883Tg	standard conditions	Figure 2 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, ameliorated	snai1b^{bns351/bns351}; bns193Tg + MO1-tnnt2a	standard conditions	Figure 1 from Gentile et al., 2021
heart contraction arrested, abnormal	snai1b^{bns351/bns351}; bns193Tg + MO1-tnnt2a	standard conditions	Figure 1 from Gentile et al., 2021
cardiac muscle cell protruding out of myocardium, ameliorated	snai1b^{bns351/bns351}; bns555Tg	standard conditions	Figure 1 supplement 1 from Gentile et al., 2021

Citations