CRISPR

CRISPR1-mydgf

ID

ZDB-CRISPR-220318-2

Name

CRISPR1-mydgf

Previous Names

None

Target

mydgf (1)

Sequence

5' - AGACGGCCGTGGGTGGACAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uwm49	mydgf

Expression

Gene expression in Wild Types + CRISPR1-mydgf

No data available

Phenotype

Phenotype resulting from CRISPR1-mydgf

No data available

Phenotype of all Fish created by or utilizing CRISPR1-mydgf

Phenotype	Fish	Conditions	Figures
otic vesicle neutrophil migration increased frequency, abnormal	mydgf^uwm49/uwm49	standard conditions	Fig. 1 from Houseright et al., 2021
whole organism Ab1-mydgf labeling decreased amount, abnormal	mydgf^uwm49/uwm49	standard conditions	Fig. 1 from Houseright et al., 2021
caudal fin neutrophil migration increased frequency, abnormal	mydgf^uwm49/uwm49	heat shock	Fig. 2 from Houseright et al., 2021
whole organism egln3 expression increased amount, abnormal	mydgf^uwm49/uwm49	heat shock	Fig. 5 from Houseright et al., 2021
whole organism egln3 expression increased amount, abnormal	mydgf^uwm49/uwm49	transection: caudal fin	Fig. 5 from Houseright et al., 2021
caudal fin neutrophil migration increased frequency, abnormal	mydgf^uwm49/uwm49; uwm4Tg/uwm4Tg	lighting conditions, transection: caudal fin	Fig. 4 from Houseright et al., 2021
caudal fin behavioral response to wounding increased duration, abnormal	mydgf^uwm49/uwm49; uwm4Tg/uwm4Tg	lighting conditions, heat shock	Fig. 4 from Houseright et al., 2021
caudal fin behavioral response to wounding increased duration, abnormal	mydgf^uwm49/uwm49; uwm4Tg/uwm4Tg	lighting conditions, transection: caudal fin	Fig. 4 from Houseright et al., 2021
caudal fin behavioral response to wounding increased duration, abnormal	mydgf^uwm49/uwm49; uwm7Tg/uwm7Tg	transection: caudal fin	Fig. 3 from Houseright et al., 2021
caudal fin neutrophil migration increased frequency, abnormal	mydgf^uwm49/uwm49; uwm7Tg/uwm7Tg	transection: caudal fin	Fig. 3, Fig. 5 from Houseright et al., 2021
caudal fin neutrophil migration decreased frequency, ameliorated	mydgf^uwm49/uwm49; uwm7Tg/uwm7Tg + MO1-arnt	transection: caudal fin	Fig. 5 from Houseright et al., 2021

Citations