CRISPR

CRISPR7-tnfa

ID
ZDB-CRISPR-220128-12
Name
CRISPR7-tnfa
Previous Names
None
Target
Sequence
5' - GCTCCTGCGTGCAGATTGAG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "CGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
ue106 tnfa
Expression
Gene expression in Wild Types + CRISPR7-tnfa
No data available
Phenotype
Phenotype resulting from CRISPR7-tnfa
No data available
Phenotype of all Fish created by or utilizing CRISPR7-tnfa
Phenotype Fish Conditions Figures
neuron ab1-elavl labeling decreased amount, abnormal tnfaue106/ue106 transection: spinal cord Fig. 4 with image from Cavone et al., 2021
spinal cord split, abnormal zf148Tg + CRISPR7-tnfa (WIK) transection: spinal cord Fig 2 with image from Keatinge et al., 2021
spinal cord neuron projection regeneration delayed, abnormal zf148Tg + CRISPR7-tnfa (WIK) transection: spinal cord Fig 2 with image from Keatinge et al., 2021
spinal cord split, abnormal tnfaue106/ue106; zf148Tg (WIK) transection: spinal cord Fig 2 with image from Keatinge et al., 2021
spinal cord neuron projection regeneration delayed, abnormal tnfaue106/ue106; zf148Tg (WIK) transection: spinal cord Fig 2 with image from Keatinge et al., 2021
Citations
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.