CRISPR

CRISPR1-nampt1

ID

ZDB-CRISPR-220106-13

Name

CRISPR1-nampt1

Previous Names

None

Target

nampt1 (1)

Sequence

5' - ACGACAAGACGGTCTTCTATGGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
pc41	nampt1
t10pm	nampt1

Expression

Gene expression in Wild Types + CRISPR1-nampt1

No data available

Phenotype

Phenotype resulting from CRISPR1-nampt1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-nampt1

Phenotype	Fish	Conditions	Figures
whole organism kdrl expression decreased amount, abnormal	nampt1^t10pm/t10pm; cz3331Tg	standard conditions	Fig. 4 from Pomreinke et al., 2024
whole organism klf1 expression decreased amount, abnormal	nampt1^t10pm/t10pm; cz3331Tg	standard conditions	Fig. 4 from Pomreinke et al., 2024
whole organism NAD(+) decreased amount, abnormal	nampt1^t10pm/t10pm; cz3331Tg	control	Fig. 2 from Pomreinke et al., 2024
whole organism NAD(+) decreased amount, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
whole organism kdrl expression decreased amount, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 4 from Pomreinke et al., 2024
whole organism klf1 expression decreased amount, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 4 from Pomreinke et al., 2024
whole organism decreased length, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
yolk broken, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
somite shape, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
notochord inner cell increased size, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
head decreased size, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
extension morphology, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
eye decreased size, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
post-vent region curved, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
notochord inner cell vacuolated, abnormal	nampt1^t10pm/t10pm; cz3331Tg + MO1-nampt1	control	Fig. 2 from Pomreinke et al., 2024
skeletal muscle satellite cell cell division decreased process quality, abnormal	c264Tg/c264Tg; gl25Tg/gl25Tg; gl36Tg/gl36Tg + CRISPR1-nampt1	physical alteration: skeletal muscle	Fig. 4 from Ratnayake et al., 2021
somite positive regulation of skeletal muscle tissue regeneration decreased process quality, abnormal	c264Tg/c264Tg; gl25Tg/gl25Tg; gl36Tg/gl36Tg + CRISPR1-nampt1	physical alteration: skeletal muscle	Fig. 4 from Ratnayake et al., 2021
skeletal muscle GFP expression decreased amount, abnormal	c264Tg/c264Tg; gl25Tg/gl25Tg; gl36Tg/gl36Tg + CRISPR1-nampt1	physical alteration: skeletal muscle	Fig. 4 from Ratnayake et al., 2021

Citations