CRISPR

CRISPR1-crtap

ID

ZDB-CRISPR-210201-5

Name

CRISPR1-crtap

Previous Names

None

Target

crtap (1)

Sequence

5' - GGAGAGCGTGGAGTTTCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
upv1	crtap

Expression

Gene expression in Wild Types + CRISPR1-crtap

No data available

Phenotype

Phenotype resulting from CRISPR1-crtap

No data available

Phenotype of all Fish created by or utilizing CRISPR1-crtap

Phenotype	Fish	Conditions	Figures
osteoblast endoplasmic reticulum cisternal network increased size, abnormal	crtap^upv1/upv1	standard conditions	Fig. 6 from Tonelli et al., 2020
notochord bone mineralization delayed, abnormal	crtap^upv1/upv1	standard conditions	Fig. 3 from Tonelli et al., 2020
vertebra misaligned with vertebra, abnormal	crtap^upv1/upv1	standard conditions	Fig. 4 from Tonelli et al., 2020
vertebra antero-posteriorly flattened, abnormal	crtap^upv1/upv1	standard conditions	Fig. 4 from Tonelli et al., 2020
vertebral column deformed, abnormal	crtap^upv1/upv1	standard conditions	Fig. 4 from Tonelli et al., 2020
vertebral column antero-posteriorly flattened, abnormal	crtap^upv1/upv1	standard conditions	Fig. 4 from Tonelli et al., 2020
whole organism decreased length, abnormal	crtap^upv1/upv1	standard conditions	Fig. 3 from Tonelli et al., 2020
vertebral body end plate malformed, abnormal	crtap^upv1/upv1	standard conditions	Fig. 7 from Tonelli et al., 2020
ceratohyal bone bone mineralization delayed, abnormal	crtap^upv1/upv1	standard conditions	Fig. 3 from Tonelli et al., 2020
head increased ratio whole organism, abnormal	crtap^upv1/upv1	standard conditions	Table 1 from Tonelli et al., 2020
chondroblast endoplasmic reticulum cisternal network increased size, abnormal	crtap^upv1/upv1	standard conditions	Fig. 6 from Tonelli et al., 2020
hyomandibula bone mineralization delayed, abnormal	crtap^upv1/upv1	standard conditions	Fig. 3 from Tonelli et al., 2020
notochord keratinous, abnormal	crtap^upv1/upv1	standard conditions	Fig. 7 from Tonelli et al., 2020
whole organism decreased length, abnormal	crtap^upv1/+	standard conditions	Fig. 3 from Tonelli et al., 2020

Citations