CRISPR

CRISPR1-stim2b

ID

ZDB-CRISPR-210105-10

Name

CRISPR1-stim2b

Previous Names

None

Target

stim2b (1)

Sequence

5' - GGACCAGCACATCACGGTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
waw301	stim2b

Expression

Gene expression in Wild Types + CRISPR1-stim2b

No data available

Phenotype

Phenotype resulting from CRISPR1-stim2b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-stim2b

Phenotype	Fish	Conditions	Figures
swimming increased linear velocity, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 3, Fig. 5 from Wasilewska et al., 2020
whole organism anxa3a expression increased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
response to light stimulus increased occurrence, exacerbated	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: L-glutamate(1-)	Fig. 7 from Wasilewska et al., 2020
whole organism anxa1c expression increased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
whole organism gpr39 expression increased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
thigmotaxis increased rate of occurrence, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 3 from Wasilewska et al., 2020
whole organism stim2b expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 1 from Wasilewska et al., 2020
response to light stimulus increased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: pentetrazol	Fig. 6 from Wasilewska et al., 2020
whole organism orai1a expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 1 from Wasilewska et al., 2020
whole organism stim2a expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 1 from Wasilewska et al., 2020
swimming increased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	control	Fig. 6, Fig. 7 from Wasilewska et al., 2020
startle response increased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: pentetrazol	Fig. 6 from Wasilewska et al., 2020
swimming occurrence, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 4, Fig. 5 from Wasilewska et al., 2020
whole organism anxa5a expression increased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
negative phototaxis increased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 4 from Wasilewska et al., 2020
whole organism ngdn expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
whole organism smc1a expression increased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
whole organism orai2 expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 1 from Wasilewska et al., 2020
whole organism rrm2 expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
swimming increased occurrence, exacerbated	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: pentetrazol	Fig. 6 from Wasilewska et al., 2020
swimming increased linear velocity, abnormal	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: L-glutamate(1-)	Fig. 7 from Wasilewska et al., 2020
phototaxis disrupted, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 4 from Wasilewska et al., 2020
swimming increased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: pentetrazol	Fig. 6 from Wasilewska et al., 2020
whole organism per1a expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
exploration behavior decreased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 3 from Wasilewska et al., 2020
swimming increased occurrence, exacerbated	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: L-glutamate(1-)	Fig. 7 from Wasilewska et al., 2020
whole organism ecrg4b expression increased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
swimming increased linear velocity, abnormal	stim2b^{waw301/waw301} (AB)	chemical treatment by environment: pentetrazol	Fig. 6 from Wasilewska et al., 2020
whole organism homer2 expression decreased amount, abnormal	stim2b^{waw301/waw301} (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
response to light stimulus increased occurrence, abnormal	stim2b^{waw301/waw301} (AB)	control	Fig. 7 from Wasilewska et al., 2020
periventricular grey zone calcium-mediated signaling amplitude, abnormal	stim2b^{waw301/waw301}; a4598Tg (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020
periventricular grey zone calcium-mediated signaling process quality, abnormal	stim2b^{waw301/waw301}; a4598Tg (AB)	standard conditions	Fig. 2 from Wasilewska et al., 2020

Citations