CRISPR

CRISPR2-grin1b

ID

ZDB-CRISPR-201201-2

Name

CRISPR2-grin1b

Previous Names

None

Target

grin1b (1)

Sequence

5' - GAGGATTTTAGGTATGGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sbu93	grin1b
sbu94	grin1b
sbu95	grin1b

Expression

Gene expression in Wild Types + CRISPR2-grin1b

No data available

Phenotype

Phenotype resulting from CRISPR2-grin1b

No data available

Phenotype of all Fish created by or utilizing CRISPR2-grin1b

Phenotype	Fish	Conditions	Figures
swimming behavior occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 7, Fig. 9 from Zoodsma et al., 2020
eating behavior decreased occurrence, exacerbated	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 6 from Zoodsma et al., 2020
response to absence of light delayed, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	light duration	Fig. 6 from Zoodsma et al., 2020
swimming decreased duration, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	light duration	Fig. 8 from Zoodsma et al., 2020
whole organism viability, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 5 from Zoodsma et al., 2020
swimming increased duration, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 8 from Zoodsma et al., 2020
response to absence of light decreased occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 7 from Zoodsma et al., 2020
habituation decreased efficacy, exacerbated	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	acoustic radiation	Fig. 10 from Zoodsma et al., 2020
swimming increased linear velocity, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 8 from Zoodsma et al., 2020
larval feeding behavior disrupted, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 6 from Zoodsma et al., 2020
locomotory exploration behavior spatial distribution of a process, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	standard conditions	Fig. 9 from Zoodsma et al., 2020
response to absence of light decreased occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	light duration	Fig. 6, Fig. 8 from Zoodsma et al., 2020
startle response increased occurrence, abnormal	grin1a^sbu90/sbu90; grin1b^sbu94/sbu94	acoustic radiation	Fig. 10 from Zoodsma et al., 2020

Citations