CRISPR

CRISPR2-thraa

ID

ZDB-CRISPR-200129-2

Name

CRISPR2-thraa

Previous Names

None

Target

thraa (1)

Sequence

5' - GTGTGCGGAGACAAGGCCAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
vp33rc1	thraa

Expression

Gene expression in Wild Types + CRISPR2-thraa

No data available

Phenotype

Phenotype resulting from CRISPR2-thraa

No data available

Phenotype of all Fish created by or utilizing CRISPR2-thraa

Phenotype	Fish	Conditions	Figures
eye all-trans-3,4-didehydroretinol increased amount, abnormal	thraa^{vp33rc1/vp33rc1}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye cyp27c1 expression increased amount, abnormal	thraa^{vp33rc1/vp33rc1}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye cyp27c1 expression increased amount, abnormal	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye all-trans-3,4-didehydroretinol increased amount, abnormal	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye cyp27c1 expression increased amount, abnormal	thraa^{vp33rc1/vp33rc1}; thrb^{stl627/stl627}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye all-trans-3,4-didehydroretinol increased amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrb^{stl627/stl627}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye all-trans-3,4-didehydroretinol normal amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}; thrb^{stl627/stl627}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
eye cyp27c1 expression amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}; thrb^{stl627/stl627}	chemical treatment by environment: thyroid hormone	Fig. 2 from Volkov et al., 2020
xanthophore decreased amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}; wprt8Tg (AB)	chemical treatment by environment: metronidazole	Figure 7 from Saunders et al., 2019
melanocyte amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}; wprt8Tg (AB)	chemical treatment by environment: metronidazole	Figure 7 from Saunders et al., 2019
xanthophore amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}; thrb^{vp34rc1/vp34rc1}; wprt8Tg (AB)	chemical treatment by environment: metronidazole	Figure 7 from Saunders et al., 2019
melanocyte amount, ameliorated	thraa^{vp33rc1/vp33rc1}; thrab^{vp31rc1/vp31rc1}; thrb^{vp34rc1/vp34rc1}; wprt8Tg (AB)	chemical treatment by environment: metronidazole	Figure 7 from Saunders et al., 2019

Citations