CRISPR

CRISPR2-lca5

ID

ZDB-CRISPR-200110-2

Name

CRISPR2-lca5

Previous Names

None

Target

lca5 (1)

Sequence

5' - GGACTTGCATGAAGATAATC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3156	lca5

Expression

Gene expression in Wild Types + CRISPR2-lca5

No data available

Phenotype

Phenotype resulting from CRISPR2-lca5

No data available

Phenotype of all Fish created by or utilizing CRISPR2-lca5

Phenotype	Fish	Conditions	Figures
retinal outer nuclear layer opn1lw1 expression mislocalised, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 6 from Qu et al., 2019
retinal rod cell rod photoreceptor outer segment degenerate, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 5 from Qu et al., 2019
red sensitive photoreceptor cell cone photoreceptor outer segment decreased length, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. S5 from Qu et al., 2019
green sensitive photoreceptor cell cone photoreceptor outer segment degenerate, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 5 from Qu et al., 2019
retinal outer plexiform layer opn1lw1 expression mislocalised, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 6 from Qu et al., 2019
retina detection of electrical stimulus involved in electroception disrupted, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 2 from Qu et al., 2019
green sensitive photoreceptor cell cone photoreceptor outer segment decreased length, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. S5 from Qu et al., 2019
photoreceptor cell cilium ift88 expression increased amount, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 8 from Qu et al., 2019
red sensitive photoreceptor cell cone photoreceptor outer segment degenerate, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 5 from Qu et al., 2019
eye decreased functionality, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 2 from Qu et al., 2019
retinal cone cell cone photoreceptor outer segment degenerate, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. S6 from Qu et al., 2019
blue sensitive photoreceptor cell cone photoreceptor outer segment decreased length, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. S5 from Qu et al., 2019
retinal cone cell degeneration, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 3 from Qu et al., 2019
photoreceptor outer segment layer rod photoreceptor outer segment disorganized, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 4, Fig. S4 from Qu et al., 2019
blue sensitive photoreceptor cell cone photoreceptor outer segment degenerate, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 5 from Qu et al., 2019
retinal photoreceptor layer morphology, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 3 from Qu et al., 2019
UV sensitive photoreceptor cell cone photoreceptor outer segment decreased length, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. S5 from Qu et al., 2019
eye photoreceptor cell cilium ift88 expression increased distribution, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 8 from Qu et al., 2019
eye photoreceptor cell photoreceptor inner segment gnat1 expression mislocalised, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 7 from Qu et al., 2019
photoreceptor outer segment layer cone photoreceptor disc membrane fragmented, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 4 from Qu et al., 2019
red sensitive photoreceptor cell photoreceptor inner segment opn1lw1 expression mislocalised, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 6 from Qu et al., 2019
UV sensitive photoreceptor cell cone photoreceptor outer segment degenerate, abnormal	lca5^{zf3156/zf3156}	standard conditions	Fig. 5 from Qu et al., 2019

Citations