CRISPR

CRISPR1-tnni1b

ID

ZDB-CRISPR-190528-2

Name

CRISPR1-tnni1b

Previous Names

None

Target

tnni1b (1)

Sequence

5' - GGAGGAGTTAGAACAAGAGCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf3081	tnni1b
zf3082	tnni1b

Expression

Gene expression in Wild Types + CRISPR1-tnni1b

No data available

Phenotype

Phenotype resulting from CRISPR1-tnni1b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-tnni1b

Phenotype	Fish	Conditions	Figures
heart myl7 expression spatial pattern, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 3 from Cai et al., 2018
myocardium bmp4 expression increased distribution, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4 from Cai et al., 2018
myocardium bmp4 expression spatial pattern, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4 from Cai et al., 2018
whole organism tnnt2a expression decreased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 1 from Cai et al., 2018
heart separated from pericardium, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 3 from Cai et al., 2018
heart nppa expression spatial pattern, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 3 from Cai et al., 2018
cardiac ventricle morphogenesis process quality, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
heart wif1 expression increased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 6 from Cai et al., 2018
heart valve absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 3 from Cai et al., 2018
endocardium spp1 expression absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4 from Cai et al., 2018
whole organism tnni1b expression decreased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 1 from Cai et al., 2018
myocardium vcana expression increased distribution, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4 from Cai et al., 2018
endocardium notch1b expression absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4 from Cai et al., 2018
heart tnni1b expression absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 1 from Cai et al., 2018
whole organism tnnc1a expression decreased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 1 from Cai et al., 2018
pericardium edematous, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
heart dkk1a expression increased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 6 from Cai et al., 2018
atrioventricular canal bmp4 expression absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4, Fig. 7 from Cai et al., 2018
myocardium vcana expression spatial pattern, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4 from Cai et al., 2018
heart tnni1b expression decreased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 6 from Cai et al., 2018
heart muscle cell sparse, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
heart lef1 expression decreased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 6 from Cai et al., 2018
endocardium has2 expression absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 4, Fig. 7 from Cai et al., 2018
heart tcf3a expression decreased amount, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 6 from Cai et al., 2018
heart contraction decreased rate, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
blood circulation decreased process quality, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
heart looping decreased process quality, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
whole organism decreased life span, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 2 from Cai et al., 2018
myocardium vcana expression absent, abnormal	tnni1b^{zf3081/zf3081}	standard conditions	Fig. 7 from Cai et al., 2018
atrioventricular ring zn-8 labeling absent, abnormal	tnni1b^{zf3081/zf3081}; s843Tg	standard conditions	Fig. 4 from Cai et al., 2018
endocardial ring EGFP expression absent, abnormal	tnni1b^{zf3081/zf3081}; s843Tg	standard conditions	Fig. 4 from Cai et al., 2018

Citations