CRISPR

CRISPR1-hsf5

ID

ZDB-CRISPR-190509-13

Name

CRISPR1-hsf5

Previous Names

None

Target

hsf5 (1)

Sequence

5' - TACAATCCCAACTTCAGACG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
sg40	hsf5
sg41	hsf5
sg42	hsf5

Expression

Gene expression in Wild Types + CRISPR1-hsf5

No data available

Phenotype

Phenotype resulting from CRISPR1-hsf5

No data available

Phenotype of all Fish created by or utilizing CRISPR1-hsf5

Phenotype	Fish	Conditions	Figures
testis hsf5 expression absent, abnormal	hsf5^sg40/sg40 (AB)	standard conditions	Fig. 1 from Saju et al., 2018
sperm sperm head increased size, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018
spermatogenesis decreased occurrence, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 2 , Fig. 3 from Saju et al., 2018
testis has extra parts of type spermatocyte, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 , Fig. 4 from Saju et al., 2018
testis hsf5 expression absent, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 1 , Fig. 2 from Saju et al., 2018
testis apoptotic process increased occurrence, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 4 from Saju et al., 2018
sperm sperm axoneme assembly decreased process quality, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018
sperm axoneme malformed, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018
sperm sperm head vacuolated, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018
testis has fewer parts of type sperm, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018
sperm sperm flagellum decreased length, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018
spermatocyte regulation of meiotic cell cycle phase transition decreased occurrence, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 4 from Saju et al., 2018
sperm sperm head plasma membrane dislocated, abnormal	hsf5^sg41/sg41 (AB)	standard conditions	Fig. 3 from Saju et al., 2018

Citations