CRISPR

CRISPR1-mdka

ID

ZDB-CRISPR-190131-2

Name

CRISPR1-mdka

Previous Names

None

Target

mdka (1)

Sequence

5' - GGCAGCTGCGTGGCCAATAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mi5001	mdka

Expression

Gene expression in Wild Types + CRISPR1-mdka

No data available

Phenotype

Phenotype resulting from CRISPR1-mdka

No data available

Phenotype of all Fish created by or utilizing CRISPR1-mdka

Phenotype	Fish	Conditions	Figures
regenerating tissue retina ccnd1 expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
regenerating tissue retina mdka expression absent, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 1. from Nagashima et al., 2019
retinal cone cell regeneration decreased occurrence, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 4. from Nagashima et al., 2019
retina cell population proliferation increased occurrence, abnormal	mdka^{mi5001/mi5001} (AB)	control	Figure 1. from Nagashima et al., 2019
retinal inner nuclear layer zrf-1 labeling increased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 5. from Nagashima et al., 2019
retina stat3 expression increased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 7. from Nagashima et al., 2019
Muller cell G1/S transition of mitotic cell cycle arrested, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
regenerating tissue retina ccne1 expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
regenerating tissue retina stat3 expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 7. from Nagashima et al., 2019
regenerating tissue retina stat3 expression increased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 7. from Nagashima et al., 2019
regenerating tissue retina ccna2 expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
retina mdka expression absent, abnormal	mdka^{mi5001/mi5001} (AB)	control	Figure 1. from Nagashima et al., 2019
regenerating tissue retinal cone cell decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 4. from Nagashima et al., 2019
whole organism decreased length, abnormal	mdka^{mi5001/mi5001} (AB)	control	Figure 1. from Nagashima et al., 2019
retinal outer nuclear layer cell population proliferation decreased occurrence, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 2. , Figure 3. from Nagashima et al., 2019
regenerating tissue retina cdk6 expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
retinal inner nuclear layer cell population proliferation decreased occurrence, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 2. , Figure 3. from Nagashima et al., 2019
whole organism decreased pigmentation, abnormal	mdka^{mi5001/mi5001} (AB)	control	Figure 1. from Nagashima et al., 2019
Muller cell cell body zrf-1 labeling increased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 5. from Nagashima et al., 2019
regenerating tissue retina ascl1a expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 7. from Nagashima et al., 2019
regenerating tissue retina cdk4 expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
retina layer formation delayed, abnormal	mdka^{mi5001/mi5001} (AB)	control	Figure 1. from Nagashima et al., 2019
regenerating tissue retina rbl2 expression increased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
eye decreased size, abnormal	mdka^{mi5001/mi5001} (AB)	control	Figure 1. from Nagashima et al., 2019
regenerating tissue retina lin28ab expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 7. from Nagashima et al., 2019
whole organism mdka expression absent, abnormal	mdka^{mi5001/mi5001} (AB)	standard conditions	Figure 1. from Nagashima et al., 2019
regenerating tissue retina id2a expression decreased amount, abnormal	mdka^{mi5001/mi5001} (AB)	light damage: eye photoreceptor cell	Figure 8. from Nagashima et al., 2019
regenerating tissue Muller cell GFP expression increased amount, abnormal	mdka^{mi5001/mi5001}; mi2002Tg	light damage: eye photoreceptor cell	Figure 5. from Nagashima et al., 2019
regenerating tissue Muller cell hypertrophic, abnormal	mdka^{mi5001/mi5001}; mi2002Tg	light damage: eye photoreceptor cell	Figure 5. from Nagashima et al., 2019
retinal outer nuclear layer cell population proliferation decreased occurrence, abnormal	mdka^{mi5001/mi5001}; mi2002Tg	light damage: eye photoreceptor cell	Figure 3. from Nagashima et al., 2019
retinal inner nuclear layer cell population proliferation decreased occurrence, abnormal	mdka^{mi5001/mi5001}; mi2002Tg	light damage: eye photoreceptor cell	Figure 3. from Nagashima et al., 2019
Muller cell cell population proliferation decreased occurrence, abnormal	mdka^{mi5001/mi5001}; mi2002Tg	light damage: eye photoreceptor cell	Figure 3. from Nagashima et al., 2019
Muller cell cell body displaced to regenerating tissue retinal outer plexiform layer, abnormal	mdka^{mi5001/mi5001}; mi2002Tg	light damage: eye photoreceptor cell	Figure 5. from Nagashima et al., 2019

Citations