CRISPR

CRISPR9-alk

ID

ZDB-CRISPR-181015-7

Name

CRISPR9-alk

Previous Names

None

Target

Sequence

5' - GGGGTAGGAGTTACTGCACT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first two "G"s were added to the 5' end to enhance binding.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ya343	alk

Expression

Gene expression in Wild Types + CRISPR9-alk

No data available

Phenotype

Phenotype resulting from CRISPR9-alk

No data available

Phenotype of all Fish created by or utilizing CRISPR9-alk

Phenotype	Fish	Conditions	Figures
whole organism alk expression decreased amount, abnormal	alk^ya343/ya343 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
whole organism alk expression decreased amount, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
eye lacks all parts of type iridophore, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 2, Fig. 4 from Mo et al., 2017
whole organism ltk expression decreased amount, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
iridophore differentiation decreased occurrence, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
melanophore stripe morphology, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
trunk lacks all parts of type iridophore, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
caudal fin lacks all parts of type iridophore, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 2 from Mo et al., 2017

Citations