CRISPR

CRISPR2-cct2

ID
ZDB-CRISPR-180710-2
Name
CRISPR2-cct2
Previous Names
  • sgRNA02 (1)
Target
Sequence
5' - GGTGCTGGCACAGACCGTCA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
An additional "G" was added at the beginning. This CRISPR also contains a polymorphism (A/G position 1194) found in the TL strain.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
zf3018 cct2
Expression
Gene expression in Wild Types + CRISPR2-cct2
No data available
Phenotype
Phenotype resulting from CRISPR2-cct2
No data available
Phenotype of all Fish created by or utilizing CRISPR2-cct2
Phenotype Fish Conditions Figures
retina zpr-1 labeling decreased distribution, abnormal cct2zf3018/zf3018 standard conditions Fig. 3 with image from Minegishi et al., 2018
ball increased size, abnormal cct2zf3018/zf3018 standard conditions Fig. 2 with image from Minegishi et al., 2018
ciliary marginal zone cell population proliferation decreased occurrence, abnormal cct2zf3018/zf3018 standard conditions Fig. 3 with image from Minegishi et al., 2018
head decreased size, abnormal cct2zf3018/zf3018 standard conditions Fig. 2 with image from Minegishi et al., 2018
retinal ganglion cell layer zn-5 labeling decreased distribution, abnormal cct2zf3018/zf3018 standard conditions Fig. 3 with image from Minegishi et al., 2018
eye decreased size, abnormal cct2zf3018/zf3018 standard conditions Fig. 2 with image from Minegishi et al., 2018
eye organ growth growth quality of occurrent, abnormal cct2zf3018/zf3018 standard conditions Fig. 2 with image,  Fig. 3 with image from Minegishi et al., 2018
eye circular, abnormal cct2zf3018/zf3018 standard conditions Fig. 2 with image from Minegishi et al., 2018
whole organism bent, abnormal cct2zf3018/zf3018 standard conditions Fig. 2 with image from Minegishi et al., 2018
retina apoptotic process increased occurrence, abnormal cct2zf3018/zf3018 standard conditions Fig. 3 with image from Minegishi et al., 2018
lens epithelium cell population proliferation decreased occurrence, abnormal cct2zf3018/zf3018 standard conditions Fig. 3 with image from Minegishi et al., 2018
Citations
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