CRISPR

CRISPR1-bloc1s1

ID

ZDB-CRISPR-171206-2

Name

CRISPR1-bloc1s1

Previous Names

None

Target

bloc1s1 (1)

Sequence

5' - GGTGGATCATCTCAATGTAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ihb815	bloc1s1
ihb816	bloc1s1

Expression

Gene expression in Wild Types + CRISPR1-bloc1s1

No data available

Phenotype

Phenotype resulting from CRISPR1-bloc1s1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-bloc1s1

Phenotype	Fish	Conditions	Figures
swim bladder ved expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
swim bladder uninflated, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2, Fig. 6 from Chen et al., 2018
swim bladder epithelium mnx1 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 6 from Chen et al., 2018
iridophore differentiation decreased process quality, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 3 from Chen et al., 2018
swim bladder nkx2.4b expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
swim bladder development decreased process quality, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2, Fig. 6, Fig. 7, Fig. 8 from Chen et al., 2018
integument iridophore differentiation decreased occurrence, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2 from Chen et al., 2018
swim bladder spry4 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
retinal pigmented epithelium melanosome organization decreased process quality, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 3 from Chen et al., 2018
swim bladder surfactant homeostasis decreased occurrence, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
swim bladder nkx2.1 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
integument melanocyte differentiation decreased occurrence, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2 from Chen et al., 2018
swim bladder pcyt1ab expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
iridophore pigment granule organization decreased process quality, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 3 from Chen et al., 2018
swim bladder chka expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
iridophore lacks all parts of type iridophore iridosome, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 3 from Chen et al., 2018
swim bladder lef1 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
swim bladder pcyt1aa expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
melanocyte Melanin decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2 from Chen et al., 2018
swim bladder ptch2 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
swim bladder mesenchyme fgf10a expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 6 from Chen et al., 2018
retinal pigmented epithelium melanosome immature, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 3 from Chen et al., 2018
retinal pigmented epithelium melanosome malformed, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 3 from Chen et al., 2018
swim bladder mesothelial cell elovl1a expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 6 from Chen et al., 2018
swim bladder fbp2 expression increased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 7 from Chen et al., 2018
swim bladder immature, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 7 from Chen et al., 2018
whole organism bloc1s1 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2 from Chen et al., 2018
optic tectum lysosome localization decreased process quality, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 4 from Chen et al., 2018
swim bladder ptch1 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
eye iridophore differentiation decreased occurrence, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2 from Chen et al., 2018
melanocyte cellular pigmentation decreased occurrence, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 2 from Chen et al., 2018
swim bladder axin2 expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
swim bladder abca3b expression decreased amount, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 8 from Chen et al., 2018
optic tectum posterior region has extra parts of type optic tectum lysosome, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 4 from Chen et al., 2018
swim bladder surfactant homeostasis arrested, abnormal	bloc1s1^{ihb815/ihb815}	standard conditions	Fig. 6, Fig. 7 from Chen et al., 2018
melanocyte Melanin decreased amount, abnormal	bloc1s1^{ihb816/ihb816}	standard conditions	Fig. 2 from Chen et al., 2018
whole organism bloc1s1 expression decreased amount, abnormal	bloc1s1^{ihb816/ihb816}	standard conditions	Fig. 2 from Chen et al., 2018
melanocyte cellular pigmentation decreased occurrence, abnormal	bloc1s1^{ihb816/ihb816}	standard conditions	Fig. 2 from Chen et al., 2018

Citations