CRISPR

CRISPR3-mgat1b

ID

ZDB-CRISPR-160128-58

Name

CRISPR3-mgat1b

Previous Names

Z000079 (1)

Target

mgat1b (2)

Sequence

5' - GGCCGGTGAAGTGATCAGAT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

CRISPRz:Z000079 (1)

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ecu300	mgat1b

Expression

Gene expression in Wild Types + CRISPR3-mgat1b

No data available

Phenotype

Phenotype resulting from CRISPR3-mgat1b

No data available

Phenotype of all Fish created by or utilizing CRISPR3-mgat1b

Phenotype	Fish	Conditions	Figures
CaP motoneuron axon decreased branchiness, abnormal	mgat1b^{ecu300/ecu300} (AB)	control	Fig. 4 from Hatchett et al., 2024
hatching delayed, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 5 from Hatchett et al., 2024
testis alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 3 from Hatchett et al., 2024
heart alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 3 from Hatchett et al., 2024
spinal cord alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 2 , Fig. 3 from Hatchett et al., 2024
skeletal muscle sarcomere organization quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 6 from Hall et al., 2023
swim bladder alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 3 from Hatchett et al., 2024
swimming behavior decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 5 from Hall et al., 2023
whole organism alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 3 from Hall et al., 2023
heart contraction decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 6 from Hall et al., 2023
whole organism viability, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 4 from Hall et al., 2023
brain mgat1b expression decreased amount, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 3 from Hall et al., 2023
swim bladder uninflated, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 5 from Hatchett et al., 2024
brain alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 2 , Figure 3 from Hall et al., 2023
integument alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 3 from Hatchett et al., 2024
whole organism mgat1b expression decreased amount, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Figure 3 from Hall et al., 2023
thigmotaxis decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	control	Figure 5 from Hall et al., 2023
skeletal muscle alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 3 from Hatchett et al., 2024
swimming behavior process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	standard conditions	Fig. 5 from Hatchett et al., 2024
swimming behavior decreased process quality, abnormal	mgat1b^{ecu300/ecu300} (AB)	vibration	Figure 5 from Hall et al., 2023

Citations