MGC:77627

ID

ZDB-CDNA-040425-3332

Name

MGC:77627

Symbol

MGC:77627

Previous Names

• FR_5514 (1)

Type

cDNA

Location

Chr: 12 Mapping Details/Browsers

Genome Resources

None

Species

Danio rerio

Library

NIH_ZGC_7

Digest

Insert Size

3093

Cloning Site

NotI-SmaI

Vector Type

Plasmid

Vector

pExpress-1

Polymerase

T7 RNA polymerase

PCR Amplification

Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' TAATACGACTCACTATAGGG 3'
5' GGATCCATTAACCCTCACTAAAGGGAAGTTTCGCTCCGGAATTCCCGGG 3'

Suppliers

None

Quality

( Weak expression pattern )

Protocol

Thisse in situ hybridization protocol

Note

None