PUBLICATION
Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone
- Authors
- Fallah, H.P., Tovo-Neto, A., Yeung, E.C., Nóbrega, R.H., Habibi, H.R.
- ID
- ZDB-PUB-190507-13
- Date
- 2019
- Source
- Molecular and Cellular Endocrinology 492: 110440 (Journal)
- Registered Authors
- Keywords
- Flutamide (FLU), Gonadotropin-inhibitory hormone (gnih), Human chorionic gonadotropin (hCG), Spermatids (SPD), Spermatozoa (SPZ), Testosterone, Zebrafish gnih (zGnih)
- MeSH Terms
-
- Animals
- Autocrine Communication*/drug effects
- Gene Expression Regulation, Developmental/drug effects
- Gonadotropins/pharmacology
- Leydig Cells/metabolism
- Male
- Neuropeptides/genetics*
- Neuropeptides/metabolism
- Paracrine Communication*/drug effects
- Spermatids/growth & development
- Spermatids/metabolism
- Spermatogenesis*/drug effects
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 31048005 Full text @ Mol. Cell. Endocrinol.
Citation
Fallah, H.P., Tovo-Neto, A., Yeung, E.C., Nóbrega, R.H., Habibi, H.R. (2019) Paracrine/autocrine control of spermatogenesis by gonadotropin-inhibitory hormone. Molecular and Cellular Endocrinology. 492:110440.
Abstract
Control of testicular development is multifactorial and involves a number of hypothalamic, hypophyseal and peripheral hormones. Here, we investigated direct action of zebrafish gonadotropin-inhibitory hormone (zGnih) which is expressed in the testis, on spermatogenesis in zebrafish, in vitro. Treatment with zGnih at the lower doses (10 and 100 nM) inhibited gonadotropin-induced spermatids/spermatozoa (SPD/SPZ) production. However, at the highest dose (1000 nM), zGnih increased basal number of SPD/SPZ and showed paradoxical effect. The effects of zGnih on testosterone and SPD/SPZ production was blocked in the presence of androgen receptor antagonist, flutamide (FLU). A number of transcripts were also measured to better understand zGnih mechanisms of action on zebrafish spermatogenesis. Our results provide strong support for the hypothesis that locally produced zGnih is a component of the complex multifactorial system that regulates testicular development and function in adult zebrafish, in part, by changes in testicular steroidogenesis and regulation of gonadotropin-induced response.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping