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Fig. 4 Expression of nkx2.5 and nkx2.7 is initiated normally in the nascent cardiomyocytes of udu−/− embryos, but becomes reduced following heart tube formation. Dorsal view of embryos showing expression of nkx2.5 (A–F) and nkx2.7 (G–L) detected by WISH in WT (A, C, E, G, I, K) and udu−/− (B, D, F, H, J, L) embryos at 12 somites (A, B, G, H), 16 somites (C, D, I, J) and 24 hpf (E, F, K, L) stages. DamID-seq Genome browser tracks of Gon4l-Dam and GFP Control-Dam at nkx2.5 (M) and nkx2.7 (N) loci. Graph of qRT-PCR results performed on RNA extracted from hearts isolated from 36 hpf and 48 hpf WT and udu−/− embryos for nkx2.5 (O) and nkx2.7 (P) expression; results were standardized to gapdh expression. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, error bars = SEM. Scale bars represent 50 μm.
Reprinted from Developmental Biology, 462(2), Budine, T.E., de Sena-Tomás, C., Williams, M.L.K., Sepich, D.S., Targoff, K.L., Solnica-Kreze, L., Gon4l/Udu Regulates Cardiomyocyte Proliferation and Maintenance of Ventricular Chamber Identity During Zebrafish Development, 223-234, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.