FIGURE

Fig. S4

ID

ZDB-FIG-150331-35

Publication

Phng et al., 2015 - Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Junctional F-actin strengthens junction stability. Related to Figure 4. (A - D) HUVECs treated with either DMSO or 10µM SMIFH2 under high shear stress. In DMSO-treated cells, junctions at the long axis of cells exhibit linear morphology (A′) that consists of VEC, ZO1 and F-actin (black arrow, B). In SMIFH2-treated cells, intercellular gaps (*, C) appear between cells. Such junctions are characterized by a wider distribution of VEC, two ZO-1 lines and a loss of F-actin in some junctions (red arrow, D). Scale bar, 10µm. (E - G) ISV junction organization in 53hpf VECwt; Tg(fli1ep:GAL4FF)^ubs3;Tg(UAS:mRFP);Tg(UAS:VECΔC-EGFP)^ubs12 and VEC^ubs8; Tg(fli1ep:GAL4FF)^ubs3;Tg(UAS:mRFP);Tg(UAS:VECΔC-EGFP)^ubs12 embryos. Embryos were immunostained with ZO1 antibody. The number of parallel junctions along the length of ISVs expressing VECΔC-EGFP was quantified (G). Scale bar, 20µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 32, Phng, L.K., Gebala, V., Bentley, K., Philippides, A., Wacker, A., Mathivet, T., Sauteur, L., Stanchi, F., Belting, H.G., Affolter, M., Gerhardt, H., Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization, 123-32, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell